Figure 4
From: The 5′UTR in human adenoviruses: leader diversity in late gene expression
Splice sites for junction between tripartite leader 3 and genes transcribed by the major late promoter in HAdV-D37. (A) Schematic for splice site junctions in mature mRNA of genes under the control of the major late promoter (dashed box: splice site). (B) Table showing the genome region, gene name, splice site, start site, and leader length for each gene transcribed under the control of the major late promoter in HAdV-D37. A549 cells were infected with HAdV-D37 at MOI of 10, mRNA was harvested at 24 hpi, and DNA removed by treatment with DNase. cDNA was generated, and PCR performed with forward primer for TPL1 and a reverse primer for each late and E3 gene. PCR products were gel purified and sequenced. Notably, mRNAs for the E3 genes RID-α and RID-β showed the same splice site, resulting in one mRNA for both genes, consistent with a polycistronic mRNA.
