Figure 5
From: The 5′UTR in human adenoviruses: leader diversity in late gene expression
Putative “j”-leader located within the CR1-α E3 gene. (A) Schematic for the location of a newly detected leader (“j”-leader) embedded within the E3 CRI-α gene, experimentally determined to be spliced to some, but not all mRNAs of the E3 genes. (B) Gel photomicrograph of mRNA transcripts amplified with forward primer from TPL1 and reverse primers from CR1-γ, CR1-β, and RID-α. Primers were chosen to elicit similarly sized bands to facilitate subsequent sequencing. (C) Nucleotide sequence of the PCR product for CR1-β. The putative j-leader sequence and splice sites are shown in yellow and green, respectively. Note an additional 4 nucleotide 5′UTR (AACC) prior to the CR1-β start site (red). The 5′UTR in (C) prior to the splice site for the j-leader is from TPL3.
