Figure 7
From: Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
Simultaneous staining of CML cells for BCR-ABL1 and for CD34. (A) The image displays simultaneous immunofluorescence staining for CD34 during BCR-ABL1 PLA-flow in a newly diagnosed CML patient. Three distinct populations were observed: A major population negative for both BCR-ABL1 and CD34, one subpopulation (13.8%) expressing BCR-ABL1 but not CD34, and one subpopulation (1.8%) positive for both CD34 and BCR-ABL1. (B) The same experiment was performed on a healthy individual as a negative control. The X-axes represent intensity of staining against BCR-ABL1 and the Y-axes reflect the CD34 staining.
