Figure 3 | Scientific Reports

Figure 3

From: 20S immunoproteasomes remove formaldehyde-damaged cytoplasmic proteins suppressing caspase-independent cell death

Figure 3

Genotoxic signaling in H460 cells. Cells were treated with FA for 3 h except for panel D. LMP7-i (0.5 μM) was present during and after FA treatments (p-CHK2, p-CHK1, p-KAP1, p-p53: phosphorylated forms). (A) Genotoxic signaling at 0 h or (B) 4 h recovery after FA exposure. (C) Westerns for markers of p53 activation at 4 h recovery post-FA. (D) Phosphorylation of CHK2 and p53 in cells treated with FA for 1 or 2 h. (E) Percentage of cells containing γ–H2AX foci (γ–H2AX+) and both γ–H2AX foci and EdU staining (γ–H2AX+/EdU+) (means ± SD, n = 3). (F) As panel E except that cells were fixed at 3 h post-FA exposure (means ± SD, n = 3).

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