Figure 6 | Scientific Reports

Figure 6

From: 20S immunoproteasomes remove formaldehyde-damaged cytoplasmic proteins suppressing caspase-independent cell death

Figure 6

HSF1 activation in cells with inhibited i-proteasomes. Cells were treated with FA in the absence or presence of 0.3 μM LMP7-i. (A) Westerns for HSF1 and its S326-phosphorylated form (p-HSF1) in whole cell lysates of IMR90 treated with FA for 1 h. (B) S326-phosphorylated HSF1 in the nuclear fraction of IMR90 cells. (C) Nuclei-bound phospho-S326-HSF1 in H460 cells treated with FA for 2 h. (D) Nuclear HSF1 in FA-treated H460 and IMR90 cells. Cells were extracted with 0.2% Triton X-100 in PBS for 10 min at 4 °C before fixation.

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