Figure 8
Ubiquitin reserves and protein polyubiquitination. Panels A–C: whole cell lysates were used for westerns and LMP7-i was added at 0.3 μM. (A) Free ubiquitin in cells treated with FA for 1 h with and without LMP7-i. (B) Free ubiquitin in H460 cells treated with FA+/−LMP7-i for 2 h and (C) IMR90 cells treated with FA+/−LMP7-i for 3 h. (D) K48-linked (K48-Ubn) and overall polyubiquitination (anti-Ub) of cytoplasmic proteins in IMR90 cells treated with FA+/−LMP7-i (0.2 μM) for 6 h.
