Figure 5
Amperometric assay experiment using the modified assay components measured with the PCB-based sensor. (A) Amperometric measurement using the PCB-based sensor and in-house bioinstrumentation platform. (B) Colorimetric validation of the experimental results shown in (A). (A,B) Show three discrete IFNγ titration assays as described in Fig. 1 with amperometric values measured on three discrete sensors and colorimetric values measured on one sensor (Promega Glomax). (C) Amperometric data for low concentration IFNγ assay using Log1.5 analyte dilutions. (D) Colorimetric verification of the experimental results shown in (C). Although the colorimetric system demonstrates a LoD of 35 pg/mL for the specific experiment, the amperometric LoD is significantly lower at ~10 pg/mL. Judging from the obtained current ranges and given the characteristics of the bioinstrumentation board, these measurements predict that the LoD could potentially reduce even more in the final optimised system.
