Figure 1
FND applications in this study. (a) Representation of FND40 uptake by macrophages. The diamond particles are phagocytosed by the macrophages and are transported in intracellular vesicles. (b) Immunolabeling approach: (1) the extracellular domain of EpCAM, with an intracellular GFP domain, is targeted by a monoclonal antibody (MOC-31); (2) biotinylated rabbit anti mouse IgG is used as a linker for labeling with (3) streptavidin-conjugated FND70 particles. (c) Schematic overview of the sample area of a diamond magnetometer for intracellular sensing. The cells, which contain diamond nanoparticles, are in a glass bottom petri dish. A microwire in close proximity is used to excite in the microwave regime. Simultaneously, fluorescence is collected through a microscope objective and a subsequent confocal microscope. (d) Schematic overview of integrated light and scanning EM and cathodoluminescence. (0) Primary incident electrons generate (1) backscattered electrons (BSE) and (2) secondary electrons (SE) which can be imaged in a SEM with the respective detectors. Also photons can be generated upon electron beam excitation called (3) cathodoluminescence (CL). Via an optical lens, these photons can be detected with for example a CCD camera or photo multiplier tube (PMT). Furthermore, with an integrated light and electron microscope (4) regular fluorescence imaging can be performed with photon excitation.
