Figure 3

LPS regulated glycoproteins in CD14⁺ monocytes and THP-1 cells. (A,B) Volcano plot (A) and heat map (B) of differentially expressed glycoproteins in CD14⁺ monocytes. (C,D) Volcano plot (C) and heat map (D) of differentially expressed glycoproteins in THP-1 cells. (A,C) Volcano plots show the t-test p-value plotted against the glycoprotein expression fold change of all identified glycoproteins in CD14⁺ monocytes and THP-1 cells at 24 h (left) and 48 h (right) of LPS treatment. Data points in lower center area of the plots (grey) display unchanged or glycoproteins with no significant fold change, whereas data points in the upper left and upper right quadrants indicate glycoproteins (red) with significant negative (left) and positive (right) changes in protein abundances, respectively. For illustration gene names are given. (B,D) Black boxes in heat maps indicate no fold change at the given time point. Green, yellow, red indicate upregulation and blue indicates down regulation according to the legend. For illustration gene names are given. (E,F) Numbers of significantly changed glycoproteins after LPS treatment in selected cellular components at different time points in CD14⁺ monocytes (E) and THP-1 cells (F); 4 h = light grey bars, 24 h = dark grey bars, and 48 h = black bars.