Figure 6 | Scientific Reports

Figure 6

From: Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression

Figure 6

TNF-alpha mRNA production in the LPS pre-stimulated tolerant state is modulated by Capric acid treatment. (A,B) Monocytes (A) and THP-1 cells (B) were pretreated for 24 h with fresh medium or 50 ng/ml LPS in fresh medium. Two hours after the initial stimulation Capric acid (500 µM) was added to some samples (CA). After 24 h cells were re-stimulated with 50 ng/ml LPS for 2 h or left untreated. RNA was isolated, reversed transcribed, and analyzed by RT-qPCR with primers specific for PPIB and TNFα. Shown are the fold changes of TNFα mRNA normalized to the house-keeping gene PPIB (monocytes) or HPRT (THP-1 cells) and compared to unstimulated control samples (mean of three independent experiments (THP-1 cells) or 5 different donors (monocytes) ± SE). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

Back to article page