Table 1 .
From: Surpassing light-induced cell damage in vitro with novel cell culture media
Primary Antibodies | |||
|---|---|---|---|
Target | Source | Species | Dilution |
NG2 | Millipore; UK | Rabbit | 1:200 |
NG2 | Millipore; UK | Mouse | 1:100 |
MBP | Serotec; UK | Rat | 1:100 |
IB4-Alexa-488* | Life-Technologies; UK | N/A | 1:100 |
Ki67 | Thermo-Scientific; UK | Rabbit | 1:200 |
β -III-Tubulin | Sigma-Aldrich; UK | Mouse | 1:100 |
GFAP | Dako: UK | Rabbit | 1:500 |
GFAP | Sigma-Aldrich; UK | Mouse | 1:500 |
Secondary Antibodies | |||
Target | Source | Fluorophore-λ ex | Dilution |
Goat-Anti-Rabbit | Life-Technologies; UK | Alexa-488 | 1:1000 |
Goat-Anti-Mouse | Life-Technologies; UK | Alexa-488 | 1:1000 |
Goat-Anti-Rat | Life-Technologies; UK | Alexa-488 | 1:1000 |
Goat-Anti-Rabbit | Life-Technologies; UK | Alexa-555 | 1:1000 |
Goat-Anti-Mouse | Life-Technologies; UK | Alexa-555 | 1:1000 |
Goat-Anti-Rabbit | Life-Technologies; UK | Alexa-647 | 1:500 |
Goat-Anti-Mouse | Life-Technologies; UK | Alexa-647 | 1:500 |
