Figure 2
Kinase-substrate prediction. (A) Heat map of secretagogue regulated kinase substrates. Kinase substrate prediction was performed using NetworKIN 3.0. High confidence regulated sites (up and down-regulated) were compared to quantified phosphorylation sites for kinase group substrates enrichment. The heat map shows over- (yellow) and underrepresentation (blue) of predicted kinase substrates among regulated sites. (B) Heatmap showing the glucose treatment temporal profiles of phosphorylation sites regulated by both GLP-1 and glucose. Only phosphorylation sites regulated by both GLP-1 in the glucose/GLP-1 synergism experiment (Exp1 in Fig. 1A) and by glucose in the glucose time course experiment (Supplementary Fig. S3A) were plotted. Kinases predicted by NetworKIN algorithm of each site are listed on the right side. Characters in red represent the reported kinase-substrate relationship.
