Figure 4
Synergistic effect at protein and pathway level. (A) Venn-diagram showing overlap of GLP-1 (LGLP1) and glucose (HGlc) regulated phosphoproteins. Only proteins with phosphorylation sites regulated by HGLP1 with high-confidence were taken into count. (B) Diagram representing different regulation pattern on the same protein mediated by GLP-1 and glucose. GLP-1 and glucose could regulated the phosphorylation status of identical or distinct sites of the same protein. (C) Ideal models of pathway level synergism. Model A means GLP-1 and glucose regulate different protein targets in the same pathway. Model B means GLP-1 and glucose choose to regulate the distinct pathways. The rectangles stand for proteins involved in the pathway. (D) Heatmap representing the number of phosphorylation sites with different regulation patterns (defined in Fig. 1G) on proteins in enriched KEGG pathways. KEGG pathway enrichment analysis was performed using DAVID algorithm with default parameters by comparing proteins possessing HGLP1 high-confidence regulated sites with proteins possessing quantified sites.
