Figure 4
From: Elevated levels of Wnt signaling disrupt thymus morphogenesis and function
E-cadherin is not required for normal thymus development. (A) Immunofluorescence analyses of thymus sections of E15.5 control (top row) or E-cadherin deficient (bottom row) embryos stained with antibodies against the indicated antigens. (B–E) Analysis of thymi from newborn control and E-cadherin-deficient mice. (B) H&E staining. (C) Staining for intracellular E-cadherin in CD45−EpCAM+ TECs from control (black tracing) or E-cadherin deficient (red tracing) mice; isotype control staining (grey tracing). (D) Total cell numbers in the thymus. (E) Proportion and absolute numbers of total TECs, proportions of Ly51+, UEA-1+ and CD80+ TECs in control (black bars) and E-cadherin-deficient (open bars) thymi. In D and E, data are depicted as mean ± SD. (F) Immunohistochemistry analyses of E15.5 E-cadherin-sufficient or -deficient Foxn1::β-catenin-transgenic embryos with antibodies against the indicated antigens.
