Figure 5
From: Elevated levels of Wnt signaling disrupt thymus morphogenesis and function
Wnt4-overexpression disrupts thymus migration and TEC differentiation. (A) Development of wild-type mice at E15.5 examined by H&E staining and RNA in situ hybridization with the indicated gene-specific probes (Gcm2 [parathyroid], Titf1 [thyroid] and Foxn1 [thymus]). (B–I) Flow cytometric analysis of TECs from newborn wild-type and Foxn1::Wnt4-transgenic mice. (B) Representative gating of EpCAM+ TECs. Gates defining UEA-1negLy51+, UEA-1+Ly51− and UEA-1negLy51neg populations are depicted; events falling outside of these gates are classified as “undefined”. (C–I) Cell numbers and proportions of various TEC subsets (wild-type, black bars; open bars, Foxn1::Wnt4-transgenic mice). Means ± SD.
