Table 12 Cellular capacity for de novo dTMP synthesis in mammals and yeast at S-phase.
| Â | Human | S. cerevesiae |
|---|---|---|
Genome size | 3.0 × 109 bp | 1.2 × 107 bp |
% AT | 59% | 61.5% |
T bases needed for replication | 1.77 × 109 molecules | 7.5 × 106 molecules |
Length of cell cycle | 24 h | 2.5 h |
Length of S-phase | 8 h | 0.83 h |
Cell volume | 8 × 10−13 L (ES cell) | 5 × 10−14 L |
dTMP synthesis rate required to replicate genome | 7.8 µM/min | 0.5 µM/min |
Measured dTMP synthesis rate (model outcomes) | 4.4 µM/min | 1.8 µM/min |
Ratio of dTMP production relative to dTMP required for replication | 0.6 | 3.6 |
