Table 3 Fluxes of the reactions catalyzed by the enzymes FTS, MTCH, MTD, MTHFR and MTR and the binding of 5mTHF and SHMT for different levels of MTHFR activity (ranging from 2x to 0.3x of wild type). CC, CT and TT refer to the C667T polymorphism.
µM/h | FTS | MTCH | MTD | MTHFR | MTR | Unbinding of SHMT and 5mTHF | ||
|---|---|---|---|---|---|---|---|---|
10fTHF → CHF | CHF → 10fTHF | CHF → CH2F | CH2F → CHF | |||||
MTHFR x2 | 13,101.4 | 332,703.8 | 330,313.4 | 37,733.2 | 35,342.8 | 26.8 | 26.8 | 17,035.2 |
MTHFR x1 - CC | 23,582.5 | 756,270.3 | 744,706.4 | 89,938.7 | 78,374.9 | 21.6 | 21.6 | 14,964.5 |
MTHFR x0.7 - CT | 31,430.7 | 884,173.6 | 864,936.0 | 106,222.3 | 86,984.7 | 16.0 | 16.0 | 12,231.1 |
MTHFR x0.5 | 36,054.6 | 954,589.4 | 930,806.0 | 115,378.8 | 91,595.3 | 11.7 | 11.7 | 9,754.6 |
MTHFR x0.3 - TT | 39,755.7 | 1,002,680.1 | 975,243.2 | 121,653.1 | 94,216.2 | 7.1 | 7.1 | 6,559.7 |
