Table 9 Fluxes of the reactions catalyzed by the enzymes PGT, AICARFT, DHFR, TYMS and SHMT and the binding/unbinding of 5 mTHF and SHMT for replete (18 µM) and low (9 µM) levels of total folate and for the CC and TT case of the C677T MTHFR polymorphism.
µM/h | PGT | AICARFT | DHFR | TYMS | SHMT | Binding of 5 mTHF and SHMT | Unbinding of 5 mTHF and SHMT | ||
|---|---|---|---|---|---|---|---|---|---|
CH2F → THF | THF → CH2F | ||||||||
CC | replete folate | 5,268.8 | 6,749.8 | 263.4 | 263.4 | 15,646.6 | 4,367.7 | 14,964.5 | 1,4964.5 |
low folate | 4,711.3 | 6,473.4 | 117.8 | 117.8 | 18,535.4 | 7,268.2 | 8150.7 | 8150.7 | |
absolute difference (% of replete folate) | 10.6 | 4.1 | 55.3 | 55.3 | 18.5 | 66.4 | 45.5 | 45.5 | |
TT | replete folate | 5,475.7 | 6,843.0 | 322.1 | 322.1 | 66,523.2 | 39,415.5 | 6,559.7 | 6,559.7 |
low folate | 4,943.9 | 6,593.4 | 120.0 | 120.0 | 73,586.0 | 51,629.1 | 5,992.1 | 5,992.1 | |
absolute difference (% of replete folate) | 9.7 | 3.6 | 62.8 | 62.8 | 10.6 | 31.0 | 8.7 | 8.7 | |
