Figure 3
Low frequency magnetic fields regulate transcription of miRNA-34a. (A) Expression level of miR-34a in LLC cells was detected using qPCR after exposure to Sham MF or MF for 2, 4 and 6 days. (B) Q-PCR analyzes levels of miR-34a in LLC cells transfected with synthetic pre-miR-34a, pre-control, anti-control and anti-miR-34a for 48 h. (C) Cell proliferation analysis of cells transfected with synthetic pre-miR-34a and pre-control using CCK-8 assay. (D,E) Cell cycle analysis of A549 and LLC cells transfected with pre-miR-34a and pre-control for 48 h using flow cytometer. (F,G) Cells treated with MF were stained with SA-β-Gal. Numbers of SA-β-Gal positive cells was calculated. (H) Q-PCR analyzes levels of miR-34a in tumor tissue of LLC mouse model (n = 6). All experiments were repeated three times. Data represent Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
