Figure 4
MiR-34a inhibits lung cancer growth via modulation of the E2F pathway. (A) Predicted miR-34a target sequence in the 3′UTR of E2F1 and E2F3. (B) Q-PCR analyzes the mRNA levels of E2F3 and E2F1 in LLC cells transfected with synthetic pre-miR-34a, pre-control, anti-control and anti-miR-34a for 48 h. (C) Western Blot analyzes E2F3 and E2F1 expression in in LLC cells transfected with pre-miR-34a and pre-control for 48 h. The numbers under each blot are relative intensity of the blot. (D) Western Blot analyzes E2F3 and E2F1 expression in LLC cells after treating with MF and Sham MF for 6 days. Relative intensity of the blot was added under each blot. (E) E2F3 and E2F1 expression in tumor tissue of LLC murine model was detected using IHC staining; Scale bars, 100 µm. (F) IHC images were calculated using Image Pro Plus software 6.0. All experiments were repeated three times. Data represent Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
