Figure 5 | Scientific Reports

Figure 5

From: LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway

Figure 5

Low frequency MF up-regulated the expression of miR-34a by stabilizing p53 protein. (A) Q-PCR analyzes the mRNA levels of P53 in LLC cells treated with MF or Sham MF. (B) Protein level of P53 in LLC cells treatment with MF or Sham MF was detected using western Blot. (C) The mRNA levels of P53 in LLC cells transfected with siRNA-P53 was detected using Q-PCR. (D) Protein level of P53 in LLC cells transfected with siRNA-P53 was detected using western Blot. Numbers under each blot are relative intensity of the blot. (E) Q-PCR analyzes the miR-34a level after treatment of siRNA-TP53 and H2O2. (F) P53 expression in tumor tissue of LLC murine model was detected using IHC staining; Scale bars, 100 µm. (G) IHC images were calculated using Image Pro Plus software 6.0 and bar graphs. All experiments were repeated three times. Data represent Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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