Figure 7
Low frequency MF stabilized p53 protein via inducing cell iron metabolism dysfunction. A549 and LLC cells were pre-incubated with medium containing FAC (100 uM) for 24 h and were exposure to MF or Sham MF for 6 days. (A) Protein level of P53 in LLC cells was detected using western blot. Numbers under each blot are relative intensity of the blot. (B) Protein expression of TfR and ferritin in LLC cells were detected using western blot. Numbers under each blot are relative intensity of the blot. (C,D) Cell proliferation of A549 cells and LLC cells was detected using CCK-8 assay. (E,F) Cell cycle of A549 and LLC cells were detected using flow cytometery. (G,H) Mean cell proportion in each phase was shown. (I,J) Representative image of SA-β-Gal staining assay was showed and numbers of SA-β-Gal positive cells was calculated. All experiments were repeated three times. Data represent Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
