Table 2 Thermodynamics of the several interactions tested by ITC.

CohScaA

Doc1bWT

2.67E7 ± 3.78E6

−10.37

−61.19 ± 0.50

−50.82

1

Doc1aWT

5.03E8 ± 2.36E8

−12.28

−38.92 ± 0.33

−26.64

1

CohScaB3 WT

Doc1b WT

1.03E7 ± 7.63E5

−9.80

−64.94 ± 0.44

−55.13

1

Doc1aWT

1.18E8 ± 2.00E7

−11, 23

−50.68 ± 0.29

−39.44

1

Doc1a I39A

3.86E6 ± 7.66E4

−9.18

−57.26 ± 0.12

−48.07

1

Doc1a S40A

1.09E8 ± 1.17E7

−11.47

−46.60 ± 0.15

−35.12

1

Doc1a V43A

1.91E6 ± 3.02E4

−8.94

−52.08 ± 0.14

−43.14

1

Doc1a Q47A

1.71E7 ± 6.89E5

−10.05

−48.27 ± 0.12

−38.21

1

Doc1a K54A

1.96E7 ± 1.28E6

−10.42

−59.73 ± 0.25

−49.30

1

Doc1a Q83A

1.54E8 ± 1.03E7

−11.71

−39.45 ± 0.83

−27.73

1

Doc1a L87A

2.81E7 ± 1.61E6

−10.53

−59.84 ± 0.19

−49.30

1

Doc1a I39A + V43A

Nb*

Nb*

Nb*

Nb*

Nb*

Doc1a V43A + Q47A

4.36E5 ± 9.66E3

−7.81

−48.79 ± 0.39

−40.98

1

CohScaB3 A38Q

Doc1aWT

Nb*

Nb*

Nb*

Nb*

Nb*

CohScaB3 N68A

1.10E8 ± 1.20E7

−11.48

−58.63 ± 0.22

−47.15

1

CohScaB3 N75A

9.09E7 ± 8.96E6

−11.10

−52.70 ± 0.17

−41.60

1

CohScaB3 K77A

4.23E8 ± 7.13E7

−12.12

−57.42 ± 0.23

−45, 30

1

CohScaB3 L79A

7.59E6 ± 3.73E5

−9.71

−51.93 ± 0.20

−42.21

1

CohScaB3 E84A

3.62E7 ± 3.45E6

−10.77

−55.45 ± 0.26

−44.68

1

CohScaB3 H121A

2.66E7 ± 1.74E6

−10.41

−52.63 ± 0.18

−42.27

1

CohScaB3 N124A

5.54E7 ± 4.68E6

−10.89

−52.80 ± 0.19

−41.90

1

CohScaB3 E84A + H121A

1.65E6 ± 2.50E5

−8.62

−66.86 ± 1.49

−58.24

1

CohScaB3 N75A + H121A

2.49E6 ± 6.66E4

−8.93

−50.84 ± 0.18

−41.90

1

CohScaB3 N75A + N124A

1.86E6 ± 3.59E5

−8.85

−56.31 ± 1.56

−47.45

1

CohScaB3 N75A + E84A

2.08E7 ± 1.29E6

−10.47

−51.76 ± 0.18

−41.29

1

CohScaB3 E84A + H121A

1.65E6 ± 2.50E5

−8.62

−66.86 ± 1.49

−58.24

1

CohScaB3 E84A + N124A

1.53E7 ± 9.83E5

−10.05

−55.04 ± 0.23

−44.99

1

CohScaB3 H121A + N124A

2.28E6 ± 9.39E4

−8.84

−46.44 ± 0.24

−37.59

1