Figure 3

Normal orientation of MII-spindles in the IVM-oocytes from XY females. (A) 2D-image of a single z-section. An MII-oocyte from an XY female stained with anti-α-tubulin antibody (green) and DAPI (blue). The white broken circle indicates the position of zona pellucida. Bar: 20 µm. (B) 3D-image reconstructed from more than 40 z-sections at every 2.0 μm. The red box indicates the boundaries of 3D-image. The white broken circle indicates the position of zona pellucida. Bar: 20 µm. (The video of the 3D-image is given in Supplementary Fig. S1) (C) Summary of spindle orientation in the oocytes from XX and XY females. The red broken circle in each picture indicates the position of zona pellucida. The vertical axis was drawn to go through the center of each oocyte and the farther pole of the spindle. The spindle orientations in individual oocytes are shown in different colors. The angle of the spindle axis was measured from the horizontal axis in each oocyte. (D) Summery of spindle angles measured in the oocytes from XX and XY females. No difference is found between the two genotypes. Data are presented as means ± SEM. The total number of oocytes examined was given in parentheses at the bottom.