Figure 3 | Scientific Reports

Figure 3

From: Moderate lifelong overexpression of tuberous sclerosis complex 1 (TSC1) improves health and survival in mice

Figure 3

Effects of TSC1 overexpression on cell signaling. Proteins extracted from different tissues were subjected to western blot analysis as described in the methods section (n > = 6 mice at 4–6 month of age for each genotype, includes both male and female mice). (a) TSC2 protein expression in different tissues. (b) mTOR signaling was monitored as the level of phosphorylation of S6K1 (threonine 389), ULK1 (serine 757), 4EBP1 (threonine 37/46), and S6 (serine 235/236). (c) The detection of autophagy related changes: Phosphorylation of ULK1 (serine 757) and the conversion of an autophagy marker LC3 from LC3I to LC3II (LC3II/LC3I ratio). WT – wild type littermates; TSC1 - Tsc1 tg mice. B – brain; L – liver; H – heart. The band intensity was normalized to wild type mice in each group. The asterisks indicate statistical significance between Tsc1 tg and wild type mice in a specific tissue (*p < 0.05).

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