Figure 8 | Scientific Reports

Figure 8

From: Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems

Figure 8

Effect of the Crc protein on the expression of a gluP’-lacZ translational fusion in P. putida. The strains used were KT2440 (wild type) and KTCRC (crc::tet) carrying plasmid pEQ424P (gluP’-lacZ). Cells were grown in LB medium and where indicated, expression of the gluP’-lacZ translational fusion was induced from the Ptrc promoter by addition of 0.5 mM IPTG. (a) Growth kinetic (measured as the turbidity at 600 nm) of each strain. (b) Activity of ß-galactosidase as a function of cell growth. (c) ß-galactosidase activity values of the gluP’-lacZ translational fusion observed at a turbidity of 0.6 (mid-exponential phase) for KT2440 or KTCRC strain. Three independent assays were performed, and a representative one is shown. Significant difference was analyzed by t-test. Statistical significance is indicated (**p < 0.01).

Back to article page