Figure 1
From: Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9
Schematic of Nasonia vitripennis embryo collection and CRISPR/Cas9 microinjections. Adult Nasonia vitripennis were mated for 4 days (i), then were supplied with a flesh fly host pupa, Sarcophaga bullata, for female parasitization for 45 minutes (ii). Embryos were then collected from the host (iii), aligned (iv), and injected with CRISPR/Cas9 components (v). Injected embryos were then gently placed back into the host (vi) for development (14 days) (vii), and when the adults emerged from the host they were subsequently screened for CRISPR/Cas9 induced mutations in target gene (viii). This entire procedure takes roughly 19 days to complete.
