Table 2 Summary of the injection and mutagenesis mediated by independent sgRNAs in N. vitripennis.
From: Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9
sgRNA | #Injected | Adult Survivors | Mosaic (%) | ||||
|---|---|---|---|---|---|---|---|
♀ | ♀ | Total (%) | M (%) | F (%) | Both (%) | ||
sgRNA-1 | 300 | 50 | 24 | 74 (25) | 16 (32) | 8 (33) | 24 (32) |
sgRNA-2 | 300 | 46 | 39 | 82 (27) | 4 (8) | 4 (11) | 8 (10) |
sgRNA-3 | 300 | 30 | 36 | 66 (22) | 10 (33) | 14 (39) | 24 (36) |
