Figure 6

Akt activator SC79 blocks PFOS-mediated MT (e.g., α-tubulin and detyrosinated α-tubulin) disorganization by attenuating disruptive distribution of EB1. Sertoli cells cultured for 3 days at a density of 0.03 × 10⁶ cells/cm². Cells were pre-treated with 2 μg/ml SC79 (5.5 µM) for 30 min, and then cells were rinsed and treated with PFOS at 20 µM for 24 hr. Thereafter, cells were fixed with ice-cold methanol and visualized by immunofluorescence microscopy using corresponding antibodies (Table 1). Consistent with data shown in Fig. 5, PFOS induced disorganization of MTs by disrupting the organization of α-tubulin and detyrosinated α-tubulin across the Sertoli cell cytosol and also +TIP EB1. However, SC79 blocked the PFOS-mediated MT disorganization, possibly by attenuating disruptive distribution of EB1 in SC79 pre-treated cells. Sertoli cell nuclei were visualized by DAPI. Scale bar, 30 µm, which applies to all other micrographs.