Table 1 Antibodies used for different experiments in this study.
Antibody | Host species | Vendor | Catalog no. | Working dilution | |
|---|---|---|---|---|---|
IB | IF/IHC | ||||
Actin | Goat | Santa Cruz Biotechnology | sc-1616 | 1:300 | |
Arp3 | Mouse | Sigma-Aldrich | A5979 | 1:3000 | 1:50 |
Akt | Rabbit | Cell Signaling Technology | 9272 | 1:1000 | |
p-Akt T308 | Rabbit | Cell Signaling Technology | 2965 | 1:1000 | |
p-Akt1 S473 | Rabbit | Cell Signaling Technology | 4060 | 1:1000 | |
p-Akt2 S474 | Rabbit | Cell Signaling Technology | 8599 | 1:1000 | |
α-tubulin | Mouse | Abcam | ab7291 | 1:500 (tissue) 1:300 (cell) | |
β-catenin | Rabbit | Thermo Fisher Scientific | 71–2700 | 1:2000 | 1:100 |
CAR | Rabbit | Santa Cruz Biotechnology | sc-15405 | 1:200 | 1:50 |
Detyrosinated α-tubulin | Rabbit | Abcam | ab48389 | 1:1000 | 1:200 |
EB1 | Mouse | BD Biosciences | 610534 | 1:200 | |
EB1 | Rabbit | Santa Cruz Biotechnology | sc-15347 | 1:200 | |
Eps8 | Mouse | BD Biosciences | 610143 | 1:5000 | 1:50 |
Formin 1 | Mouse | Abcam | ab68058 | 1:500 | 1:50 |
FAK | Mouse | Millipore | 05–537 | 1:1000 | |
p-FAK-Tyr407 | Rabbit | Thermo Fisher Scientific | 44–650G | 1:1000 | 1:50 |
N-cadherin | Mouse | Thermo Fisher Scientific | 33–3900 | 1:200 | 1:100 |
Occludin | Rabbit | Thermo Fisher Scientific | 71–1500 | 1:250 | 1:100 |
Palladin | Rabbit | Proteintech | 10853–1-AP | 1:1000 | 1:100 |
Plastin 3 | Rabbit | Abcam | ab137585 | 1:500 | |
ZO-1 | Rabbit | Thermo Fisher Scientific | 61–7300 | 1:250 | 1:100 |
Goat IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2350 | 1:3000 | |
Rabbit IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2370 | 1:3000 | |
Mouse IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2371 | 1:3000 | |
Rabbit IgG-Alexa Fluor 488 | Goat | Thermo Fisher Scientific | A-11034 | 1:250 | |
Mouse IgG-Alexa Fluor 488 | Goat | Thermo Fisher Scientific | A-11029 | 1:250 | |
