Figure 2
huBLT mice develop naïve, central memory and effector memory T-cells. huBLT mice were generated as described in Fig. 1. At 17 weeks post-humanization, total mononuclear cells from the thymic organoid (a) and liver (b) of an uninfected huBLT mouse (cohort 3) were analyzed by flow cytometry for human T-cell subsets. All samples were gated on total, viable, murine CD45−, human CD45+ leukocytes. Panel 1 shows CD4+ and CD8+ T-cell subsets (gated on CD3+ T-cells). CD4+ T-cell subsets (panels 2 and 3) can be further analyzed to assess T-cell development by staining for naïve (Tnaïve) (CD45RA + CCR7+), central memory (TCM) (CD45RA-CCR7+) and effector memory (TEM) (CD45RA-CCR7−) T-cells. Effector memory T-cells can be further classified by expression of CD45RO (panel 3). CD8+ T-cell subsets (panels 4 and 5) can be analyzed for naïve, central memory and effector memory T-cells as described above. Naïve CD8+ T-cells (CD45RA + CCR7+) can also be further classified by the expression of CD28 and CD27 (panel 6).
