Table 1 Primers sequence of reverse transcription-PCR analysis for genes.
From: Paraquat poisoning induced pulmonary epithelial mesenchymal transition through Notch1 pathway
Gene | Forward Primer | Reverse primer | Product length |
|---|---|---|---|
E-cadherin | CGTAGCAGTGACGAATGTGGTAC | AACTGGAGAACCATTGTCTGTAGC | 364 |
α-SMA | GGCTGTTTTCCCATCCATTGT | TCTTTTGCTCTGTGCTTCGT | 103 |
Notch1 | AGAGCTTTTCCTGTGTCTGTCC | CGGTACAGTCAGGTGTGTTGTT | 414 |
Notch2 | GACTGCCAATACTCGACCTC | TTCAGAAGTGAAGTCTCCAG | 438 |
Notch3 | CCTCTTTCACCTGTACCTGTCC | ACACAGTAGTGGGAGTGGTCCT | 496 |
Notch4 | CAACTCTGCGAGAACGGTGG | TGGAAGGAGCCCAAGGTGTT | 443 |
Jagged-1 | TCGCTGTATCTGTCCACCTG | AGTCACTGGCACGGTTGTAG | 227 |
TGF-β1 | TCCACCTGCAAGACTATCGAC | GAGGTATCGCCAGGAATTGTT | 456 |
Smad3 | ACCAGGGCTTTGAGGCTGTC | GCAAAGGCCCATTCAGGTG | 144 |
GAPDH | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA | 131 |
