Figure 5
From: Down-regulation of cholinergic signaling in the habenula induces anhedonia-like behavior
MHb cholinergic neurons regulate the population of 5-HT-immunoreactive neurons in the dorsal raphe nucleus. (a and b) DREADD mice expressing Cre-dependent hM3Dq were pharmacogenetically stimulated for 60 min using i.p. injection of CNO. Cell nuclei and 5-HT were visualized using Hoechst staining (blue) and Cy3 (red), respectively. CNO administration reduced the number of 5-HT-immunoreactive neurons in the DRN. Data represent mean ± SEM (PBS, n = 7 mice; CNO, n = 8; ***P < 0.001, Student’s t-test). Scale bar, 50 μm. (c and d) CHAT knockdown effect on the number of 5-HT-immunoreactive neurons in the DRN. Immunohistochemical analysis was performed using rats that were used for behavioral tests in Fig. 2. Cell nuclei and 5-HT were visualized using Hoechst staining (blue) and Cy3 (red), respectively. CHAT knockdown increased the number of 5-HT-immunoreactive neurons in the DRN Data represent mean ± SEM (sh-SCR, n = 5 rats; sh-CHAT, n = 5; **P < 0.01, Student’s t-test). Scale bar, 100 μm.
