Figure 3 | Scientific Reports

Figure 3

From: Bioinformatic Approaches Including Predictive Metagenomic Profiling Reveal Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments

Figure 3

Metabolic reconstruction and functional biomarkers of metagenomes from oil polluted habitats. Cladogram showing a subset of the 4-level KEGG BRITE hierarchical structure denoted by four rings, as inferred against KEGG metabolic modules detected by HUMAnN2 from metagenomic gene family abundance data produced by PICRUSt for all oil contaminated samples. The outermost ring represents KEGG functional modules that have been detected in at least one of the 65 PICRUSt predicted metagenomes as reconstructed by HUMAnN2, while the innermost ring represents the Level 1 KEGG BRITE clades. Differentially abundant KEGG metabolic modules inferred by LEfSe using KEGG module abundance data generated by HUMAnN2 are colored corresponding to the oil contaminated habitat they have been identified to be differentially abundant in (see legend). Circles not differentially abundant in any habitat are colorless. Brackets represent a single KEGG BRITE clade at that Level from which daughter clades originate. KEGG BRITE clades with a single daughter clade are joined using regular branches. Annotations for the KEGG BRITE hierarchy follow an outside-in pattern, wherein Level 1 KEGG BRITE clades are annotated in the outermost section of the cladogram with lower clades annotated further inside ending at the outermost circle in that section of the cladogram. More information on this style of representation can be found elsewhere28, 36, 83.

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