Figure 5

miR-19a regulates c-Met by targeting its 3′UTR. Schematic representation of the 3′UTR of c-Met with the predicted target site for miR-19a. The mutant site of c-Met 3′UTR is indicated (dotted line); (B,C) Reporter constructs containing the wild-type c-Met 3′UTR were co-transfected into Pc9 cells along with miR-19a or miR-NC (B) or Ihn-19a or Inh-NC (C); the relative luciferase activity was measured (mean ± sd, n = 3, **p < 0.01). (D,E) Reporter constructs containing the c-Met 3′UTR harboring a mutation at the predicted miR-19a target sequence were co-transfected into Pc9 cells with miR-19a or miR-NC. The same construct was co-transfected with Inh-19a or Inh-NC; the relative luciferase activity was measured (mean ± sd, n = 3, **p < 0.01).