Figure 4 | Scientific Reports

Figure 4

From: The centrosomal OFD1 protein interacts with the translation machinery and regulates the synthesis of specific targets

Figure 4

Accumulation of specific proteins in Ofd1-IND and Ksp-Cre;Pkd1 flox/flox mutant kidneys. (a) Real Time-PCR on polysomal mRNAs. The enrichment of Net1, Gdi2, Vcl, Vps39 and Gh was validated in kidneys of Ofd1-IND mutants (IND; gray bars at P8, black bars at P20) compared to controls (C; white bars) both at P8 and P20. (b–d) Transcriptional levels for Net1, Gdi2, Vcl, Vps39 and Gh were measured by Real-Time PCR on mRNA extracted from total kidneys of controls (white bars) and Ofd1-IND mice (black bars in b) and Ksp-Cre;Pkd1 flox/flox mutants (black bars in d). WB analysis showed the accumulation of all targets in renal lysates from both Ofd1-IND (c) and Ksp-Cre;Pkd1 flox/flox mutants (e). For Gdi2 and Vcl (in e) the αtubulin used for normalization come from the same blot. Data are presented as the mean ± SEM. Student’s t-test was used to calculate the p-value. ns: not significant, p-value > 0.05; *p-value < 0.05; **p-value < 0.05; ***p-value < 0.01.

Back to article page