Figure 6 | Scientific Reports

Figure 6

From: The centrosomal OFD1 protein interacts with the translation machinery and regulates the synthesis of specific targets

Figure 6

Translation initiation factors localize at the centrosome in HEK293 cells. (a) Immunofluorescence experiments demonstrate that eIF4G and eIF4E colocalize with γ, a centrosomal marker (arrows). (b) Silencing of eIF4E results in downregulation of the signal, confirming the specificity of the immunostaining of the eIF4E antibody. (c) Single channel IF are presented for each protein analysed, arrowheads indicate the staining that suggest the centrosomal localization. (d) Magnification of IF experiments showing colocalization of eIF3B, eIF3G, eIF4E, eIF4A1, eIF4G with centrosomal markers (OFD1 or γtubulin). (e) Analysis of centrosomal localization of eIF4E and eIF3B by Superresolution microscopy. IF (left panels) and 3D reconstruction (right panels) are provided. Bar = 10 μm. For Superresolution images Bar = 2 μm. For all panels n = 3.

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