Figure 1
From: Regulation of viral gene expression by duck enteritis virus UL54
Construction of DEV-ΔUL54 and DEV-ΔUL54 (Revertant). (A) Construction of DEV-ΔUL54 and DEV-ΔUL54 (Revertant). The DEV CHv-BAC-ΔUL54 and DEV CHv-BAC-ΔUL54 (Revertant) were constructed with the Red recombinant system and rescued in DEFs cells. Using the Cre-LoxP system, the BAC-EGFP tag was removed. (B) Identification of DEV-ΔUL54 and DEV-ΔUL54 (Revertant) by PCR. M: DL15000; 1: DEV CHv-BAC-ΔUL54; 2: DEV-ΔUL54; 3: DEV-ΔUL54 (Revertant); 4: DEV CHv-BAC-ΔUL54 (Revertant). The target fragment size in DEV CHv-BAC-ΔUL54/DEV CHv-BAC-ΔUL54 (Revertant) was approximately 10000 bp, and it was nearly 1700 bp for DEV-ΔUL54/DEV-ΔUL54 (Revertant). (C) Identification of DEV-ΔUL54 and DEV-ΔUL54 (Revertant) by IFA. DEFs cells that were infected with DEV-ΔUL54 or DEV-ΔUL54 (Revertant) were subjected to IFA with Anti-UL54 polyclonal antibody as a primary antibody; DEV-ΔUL54 treated with Anti-UL13 polyclonal antibody as primary antibody was used as a control. (D) Identification of DEV-ΔUL54 and DEV-ΔUL54 (Revertant) by Western blot. Proteins of DEFs cells infected with DEV-ΔUL54 or DEV-ΔUL54 (Revertant) were subjected to Western blot with Anti-UL54 polyclonal antibody as a primary antibody. 1: DEV-ΔUL54 (Revertant); 2: DEV-ΔUL54; 3: DEV-ΔUL54 subjected with Anti-UL13 polyclonal antibody as a primary antibody.
