Figure 2
From: Regulation of viral gene expression by duck enteritis virus UL54
Growth curve and plaque size of the DEV-derived viruses. (A) Growth curve. DEFs were infected with DEV-LoxP, DEV-ΔUL54 or DEV-ΔUL54 (Revertant) at a titer of 200 TCID50 and harvested at different time points. The curve was generated based on the titers of different harvests by testing TCID50. a: p < 0.05. (B) Phenotypes of viral plaques. Plaques were formed on DEFs under a semi-solid medium overlay containing methylcellulose. After fixation, crystal violet staining was performed. (C) Analysis of the plaque area. The mean areas of plaque were analyzed using IPP 6.0. **p < 0.01.
