Table 2 Comparison of widely used agglutination methods (slide, tube microplate or column/gel filtration format)8 and the presented flow retardation microfluidic assay.

From: Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system

 

Agglutination methods in general

Flow retardation microfluidic assay

Blood required

>10 µl

3 µl

Assay duration

>10 min

7 min

Quantitation

only with instrument

manually possible

Price

variable

>slide

Reverse typing

possible

not possible

Sample preparation

pipetting/mixing/centrifugation etc.

no preanalytical steps

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