Figure 4 | Scientific Reports

Figure 4

From: White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population

Figure 4

qPCR detection of WSSV in experimental animals. DNA isolated from uropods from shrimp collected at different time points before, during and after challenge was subjected to a qPCR assay detecting WSSV VP28. Cq-values from individual shrimp were compared to a standard curve, made up of 10-fold serial dilutions of a plasmid containing a VP28 insertion, to obtain absolute quantities of WSSV genome copy numbers. The Y-axis represents the absolute WSSV genome copy numbers obtained from each individual shrimp sample. Error bars indicate the 95% CI around the mean.

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