Figure 5
A VP28 mRNA specific RT-qPCR assay, without specificity for the VP28 dsRNA treatment, was applied in order to monitor WSSV replication rate during the first 60 hours of infection. The relative gene expression measured at 0 hpi arise from variation in reference gene expression and not VP28 mRNA, for which all samples collected at 0 hpi did not produce any Cq-values. Similarly, no Cq-values were obtained at 12, 36 and 60 hpi for the VP28 dsRNA treatment group. One-way ANOVA with Bonferroni’s multiple comparisons test was performed on GFP dsRNA treatment at 36 hpi in comparison to no treatment (PBS) at 36 hpi, showing significant variation at α = 0.05. Error bars indicate the 95% CI around the mean.
