Figure 7
During the first 60 hours of WSSV infection, relative gene expression on a panel of host genes associated with small RNA silencing was carried out by RT-qPCR assays (a–e). Relative gene expression was measured through fold variation from the lowest obtained normalized expression value observed in all samples. One-way ANOVA with Tukey’s multiple comparisons test was performed individually for each gene comparing groups at 36 hpi. Significant variation at α = 0.05 is indicated and error bars indicate the 95% CI around the mean. To investigate LvDcr2, LvAgo2 and LvSid-1 host genes’ dependency to WSSV virus load and time since infection, all individual normalized expression values obtained for these genes in the no treatment (PBS) and GFP dsRNA treatment groups was plotted against VP28 normalized expression level registered in the respective samples (f).
