Figure 1

Identification of TSCM CD45RO⁻ CCR7⁺ CD95⁺ CD4⁺ and CD8⁺ T cells. (A) CD4⁺ TSCM and (B) CD8⁺ TSCM cells pre- immunization, 2 and 28 weeks after the last immunization (n = 12–18), all from different immunized subjects. (C) The specificity of TSCM was examined by in vitro stimulation with HIVgp140 and compared with recombinant IL-15 and microbial (m) HSP70. (D) Shows significant increase in CD4⁺ TSCM 28 weeks after the last immunization in the vaccinated cohort, but not in the CCR7⁺ central (CM), CCR7- effector memory (EM) or naïve CD4⁺ T cells (n = 10 in each group, (E,F,G). (H) Identification of TSCM and gating.