Table 2 Necrotrophic activity of P. intermedia and DNA concentrations of dead P. gingivalis and dead S. oralis (Log (MO/mL) analyzed by qPCR (mean ± standard deviation, n = 3).

From: Necrotrophic growth of periodontopathogens is a novel virulence factor in oral biofilms

A

Living Bacteria

Dead bacteria

Log (CFU/mL) (Vitality qPCR)

Log (CFU/mL) (Standard qPCR)

P. intermedia

109 P. gingivalis

10.43 ± 0.04*

10.76 ± 0.07*

108 P. gingivalis

10.10 ± 0.10*

10.65 ± 0.03*

107 P. gingivalis

9.63 ± 0.20

9.72 ± 0.13

Control

9.36 ± 0.06

9.47 ± 0.10

P. intermedia

109 S. oralis

10.20 ± 0.09*

10.20 ± 0.11*

108 S. oralis

9.78 ± 0.08

10.12 ± 0.08*

107 S. oralis

9.71 ± 0.08

9.73 ± 0.11

Control

9.88 ± 0.05

9.68 ± 0.04

B

Living Bacteria

Dead bacteria

Initial Log ([DNA dead bacteria]/mL)

Final Log ([DNA dead bacteria]/mL)

P. intermedia

109 P. gingivalis

8.29 ± 0.06

7.89 ± 0.11#

108 P. gingivalis

7.26 ± 0.14

6.48 ± 0.24#

107 P. gingivalis

6.27 ± 0.07

5.89 ± 0.04#

Control (without Pi)

109 P. gingivalis

8.31 ± 0.06

8.15 ± 0.13*

P. intermedia

109  S. oralis

7.32 ± 0.22

5.87 ± 0.18#

108 S. oralis

6.92 ± 0.03

6.37 ± 0.35*

107 S. oralis

5.66 ± 0.33

3.67 ± 0.19*

Control (without Pi)

109 S. oralis

7.73 ± 0.44

7.55 ± 0.50*

  1. (A) Necrotrophic activity of P. intermedia in presence of dead P. gingivalis and dead S. oralis analyzed by standard and vitality qPCR. Control contains only living P. intermedia. *Designates a statistically significant outgrowth of P. intermedia respect to control (p < 0.05). (B) Initial and final concentrations of DNA from dead P. gingivalis and dead S. oralis during the necrotrophic activity of P. intermedia analyzed by standard qPCR. Control contains only DNA of dead bacteria. #Designates a statistically significant decrease of DNA from dead bacteria respect to control (p < 0.05).
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