Table 3 Vitality qPCR (Log (MO/mL) of the different species (mean ± standard deviation, n = 3) in the 14 species community in presence of living and dead bacteria in BHI-2 after 24 hours incubation.

From: Necrotrophic growth of periodontopathogens is a novel virulence factor in oral biofilms

 

Planktonic

Biofilm

BHI-2

+dead Pi

+Living Pi

+dead Pg

+Living Pg

BHI-2

+dead Pi

+Living Pi

+dead Pg

+Living Pg

Aa

7.27 ± 0.11

7.93 ± 0.10*

6.54 ± 0.07#

7.69 ± 0.05*

7.32 ± 0.08

6.88 ± 0.03

7.56 ± 0.10*

6.11 ± 0.11#

6.40 ± 0.11#

6.99 ± 0.15

Pi

7.15 ± 0.14

7.70 ± 0.11*

7.56 ± 0.07*

8.75 ± 0.06*

6.89 ± 0.04

8.16 ± 0.03

8.97 ± 0.19*

8.65 ± 0.04*

8.72 ± 0.06*

7.64 ± 0.07#

Pg

8.05 ± 0.09

8.15 ± 0.09

7.98 ± 0.05

8.83 ± 0.10*

10.56 ± 0.03*

7.81 ± 0.03

8.08 ± 0.11*

8.05 ± 0.05*

8.41 ± 0.06*

9.75 ± 0.13*

Fn

8.08 ± 0.05

9.26 ± 0.17*

8.89 ± 0.08*

10.08 ± 0.30*

9.08 ± 0.01*

8.93 ± 0.12

10.19 ± 0.13*

9.82 ± 0.18*

9.89 ± 0.11*

9.56 ± 0.14*

A. naeslundii

4.58 ± 0.09

3.28 ± 0.17#

4.98 ± 0.17

4.87 ± 0.15

4.52 ± 0.36

6.77 ± 0.05

3.94 ± 0.37#

5.95 ± 0.03#

6.82 ± 0.11

6.86 ± 0.16

A. viscosus

6.37 ± 0.15

2.95 ± 0.12#

5.75 ± 0.11#

6.27 ± 0.18

6.63 ± 0.17*

7.77 ± 0.07

4.85 ± 0.49#

7.00 ± 0.01#

7.67 ± 0.06

7.54 ± 0.12

S. mutans

7.01 ± 0.22

5.78 ± 0.12#

7.08 ± 0.21

7.27 ± 0.18*

6.09 ± 0.17#

8.09 ± 0.04

6.22 ± 0.30#

8.21 ± 0.10

7.78 ± 0.07#

7.82 ± 0.14

S. sobrinus

6.06 ± 0.15

5.66 ± 0.20#

6.07 ± 0.08

6.40 ± 0.11*

4.95 ± 0.21#

6.65 ± 0.03

6.94 ± 0.16

7.10 ± 0.13*

6.39 ± 0.08#

6.90 ± 0.25

S. sanguinis

7.78 ± 0.13

4.41 ± 0.15#

6.39 ± 0.05#

7.84 ± 0.05

6.47 ± 0.10#

8.21 ± 0.02

6.55 ± 0.37#

7.37 ± 0.07#

7.67 ± 0.07#

8.64 ± 0.14

S. gordonii

9.39 ± 0.16

8.67 ± 0.12#

7.28 ± 0.19#

9.44 ± 0.18

8.65 ± 0.06#

10.38 ± 0.05

9.17 ± 0.28#

9.43 ± 0.08#

10.06 ± 0.12#

10.33 ± 0.17

S. oralis

7.78 ± 0.07

3.93 ± 0.16#

5.61 ± 0.15#

7.35 ± 0.23

8.03 ± 0.05*

7.99 ± 0.01

5.60 ± 0.29#

6.58 ± 0.02#

7.64 ± 0.05#

8.25 ± 0.18

S. salivarius

5.98 ± 0.12

5.47 ± 0.20#

4.61 ± 0.20#

6.34 ± 0.23*

6.00 ± 0.19

7.39 ± 0.05

6.66 ± 0.35

6.02 ± 0.07#

6.76 ± 0.08#

6.90 ± 0.21

V. parvula

8.01 ± 0.07

8.65 ± 0.15*

8.51 ± 0.06*

8.26 ± 0.06*

9.01 ± 0.07*

10.33 ± 0.06

10.60 ± 0.08*

10.06 ± 0.10#

10.45 ± 0.07

10.16 ± 0.14

S. mitis

5.99 ± 0.15

2.80 ± 0.27#

4.81 ± 0.16#

6.16 ± 0.10*

5.19 ± 0.14#

6.77 ± 0.27

4.05 ± 0.21#

5.11 ± 0.06#

6.25 ± 0.03#

6.64 ± 0.08

  1. BHI-2 condition refers to a 14 species community without the addition of dead bacteria. *Designates a statistically significant increase of the bacterial concentration with respect to BHI (p < 0.05). #Designates a statistically significant decrease of the bacterial concentration in respect to BHI (p < 0.05).
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