Table 4 Primers of virulence and house-keeping genes from P. gingivalis (Pg) and P. intermedia (Pi).
From: Necrotrophic growth of periodontopathogens is a novel virulence factor in oral biofilms
Genes | Sequence | Length |
|---|---|---|
16 S rRNA (Pi) house-keeping | F: CGGTCTGTTAAGCGTGTTGTG R: CACCATGAATTCCGCATACG | 99 pb37 |
Hemagglutinin gene phg (Pi) | F: TTGCAAGTATTGGTTCGGC R: TCAGGCTGTAAGCGTAGACG | 438 pb gb|AF017417.1|:154-1083 GenBank: AF017417.1 protein_id = “AAB70257.1” |
Heat shock protein 90 htpG (Pi) | F: TGAACGTAAGCCGCAGTTAC R: TTGTTCTTGGCGCAAAGCAG | 860 pb gi|965694490:724387-726444 GenBank: AP014598.1 protein_id = “BAU19169.1” |
Chaperone dnaK gene (Pi) | F: ACGGTGTTGAGGTTGTTGGT R: CTCCGCTGACACTCGGTATC | 598 pb gi|965692493:1150302-1152203 GenBank: AP014597.1 protein_id = “BAU17563.1” |
Chaperone dnaJ gen (Pi) | F: CGAACATTGTCACGGAACAG R: GCAGGAAGTCCTTTGCCTCT | 519 pb gi|965692493:412565-413722 GenBank: AP014597.1 protein_id = “BAU16891.1” |
Chaperone groeL gen (Pi) | F: TGAAGATTTGCGCTGTCAAG R: TAGCTGGAGTTTCTTCAGGT | 780 pb gi|965692493:260345-261970 GenBank: AP014597.1 protein_id = “BAU16749.1” |
Chaperone groeS gene (Pi) | F: AGCGGAGCAGAAAGTAGGTG R: CAACAGCAAGAACGTCGCTT | 215 pb gi|965692493:259953-260222 GenBank: AP014597.1 protein_id = “BAU16748.1” |
Chaperone clpB gene (Pi) | F: CGCTCCATACGAAGCTTTGC R: ATTATCCGTGGCGACGTACC | 548 pb gi|965692493:10024-12612 GenBank: AP014597.1 protein_id = “BAU16518.1” |
Polysialic acid transport kpsD gene (Pi) | F: CGGCAGCTTTAAGTTGTGCC R: GGGTATGACCGATACGCAGG | 422 pb gi|877799833:1064644-1065294 GenBank: AP014925.1 protein_id = “BAR95643.1” |
RNA polymerase ECF-type sigma factor ecf gene (Pi) | F: ATGGAAGCCTCGCAATTCAA R: TCCGTAAGTCCTGTTTCGGC | 428 pb gi|965692493:1634435-1634935 GenBank: AP014597.1 protein_id = “BAU17984.1” |
Interpain A inpA gene (Pi) | F: GAAGGACAACTACAGCGGAAA R: TCCTTTCGTTAGTTCGCTGA | 250 pb39 |
Invasin adpC gene (Pi) | F: CTGTGTTCTTCAGTTGCACGC R: TATTGCGCCCGCCTTCACCTC | 900 pb31 |
16 S rRNA (Pg) house-keeping | F: GCGCTCAACGTTCAGCC R: CACGAATTCCGCCTGC | 68 pb40 |
Collagenase prtC gene (Pg) | F: TTCTTTGTAGCAGCGGCAGA R: CGTATGCCAACGAGATCGGA | 506 pb gi|333802964:798156-799409GenBank: AP012203.1 protein_id = “BAK24920.1” |
Arginine-specific cysteine proteinasergpA gen (Pg) | F: CACAACCTTGGCTTCGTTGG R: CAATGGCGCCAAACCTCAAA | 508 pb gi|333802964:315477-320528 GenBank: AP012203.1 protein_id = “BAK24470.1” |
Arginine-specific cysteine proteinase rgpB (Pg) | F: GGAGGAATCTCGTTGGCCAA R: TGCCGACCATATCACCATCG | 571 pb gi|333802964:1685747-1687957 GenBank: AP012203.1 protein_id = “BAK25772.1” |
Lysine specific cysteine protease kgp (Pg) | F: TGGCTTTGGTTGGTGACACT R: AGCCCCTTTCTCCTTAACGC | 581 pb gb|AF017059.1|:738-5936 GenBank: AF017059.1 protein_id = “AAC26523.1” |
FimA type II fimbrilin fimA gen (Pg) | F: TTGCAGGGGTATAAGCACCG R: TTGGAGTTGGCGATGACGAA | 567 pb gi|333802964:1331121-1332290 GenBank: AP012203.1 protein_id = “BAK25445.1” |
