Figure 7

DAC treatment inhibits the Th1 and Th17 responses. Allogeneic CD4⁺ T cells (obtained from healthy controls) were co-cultured with DAC-treated or –untreated DCs (derived from VKH patients) for 5 days. The frequencies of IFN-γ⁺ (A) and IL-17⁺ cells (B) in the CD4⁺ T cells co-cultured with DAC-treated or –untreated DCs were evaluated by flow cytometry (n = 7, *p < 0.05,**p < 0.01). (C) The representative results of the IFN-γ⁺ and IL-17⁺ cells in the CD4⁺ T cells co-cultured with DAC-treated or –untreated DCs. The protein levels of IFN-γ (D) and IL-17 (E) in the cell culture supernatants were measured by ELISA (n = 7, *p < 0.05). The data are shown as mean ± SEM. Ctr-DCs: control DCs (DAC-untreated DCs), DAC-DCs: DAC-treated DCs. Paired t test was used to compare the protein concentrations of IFN-γ and IL-17 and the frequencies of IFN-γ⁺ and IL-17⁺ cells between two groups.