Figure 1
From: Variability in bacterial flagella re-growth patterns after breakage

Broken flagella re-grow after mechanical damage. Strain EM4067 (P araBAD -fliC T237C ∆fliC) was used to assess re-growth of flagellar filaments after mechanical shearing using a 3-color in situ labeling protocol. (A) Top: F1, F2 and F3 filament fragment lengths of the control sample after 2-color labeling (left panel) and 3-color labeling (right panels). Bottom: Representative fluorescent microscopy images. Scale bar represents 2 µm. (B) Top: F1, F2 and F3 filament fragment lengths of the shearing sample after 2-color labeling (pre-shearing, left panel) and 3-color labeling (post-shearing, right panels). Bottom: Representative fluorescent microscopy images. Scale bar represents 2 µm.