Figure 2
From: Variability in bacterial flagella re-growth patterns after breakage

Formation of a new filament cap on re-grown flagellar filaments. (A–D) Representative electron microscopy images of gold-labeled flagellar filaments and newly formed FliD cap structures of strain TH22231 (P araBAD -fliD::HA fliC T237C) demonstrating that broken filaments re-grow. Flagellar filaments were gold-labeled before mechanically shearing the flagella using viscous shearing forces. Induction of hemagglutinin (HA) epitope tag-labeled FliD from the P araBAD promoter (indicated as ‘new cap’ in the figure) was induced after mechanical shearing of the filaments.